Meles, Dewa Ketut, Rachmawati, Kadek, Hamid, Iwan Sahrial, Mustofa, Imam, Wurlina, Wurlina, Suwasanti, Niluh, Putri, Desak Ketut Sekar Cempaka and Utama, Suzanita (2022) α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Veterinary Medicine International, 22 (1). pp. 1-9. ISSN e-ISSN: 20420048, p-ISSN: 20908113
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Abstract
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse e7ects of dioxin pollution exposure are needed in all aspects, including reproductive health. .is study aimed to determine the e7ect of α-tocopherol on superoxide dismutase (SOD) and malondialdehyde (MDA) levels, live spermatozoa, apoptosis, and necrosis in male rats exposed to dioxin as a model. .irty healthy 12-week-old male rats were randomly divided into 8ve groups. Rats in the control group were given corn oil twice daily at 4-hour intervals. .e remaining rats were given TCDD 700 mg/kg BW daily, followed by administration of corn oil and α-tocopherol at doses of 77, 140, and 259 mg/kg BW/d for T0, T1, T2, and T3 groups, respectively. .e treatments were conducted for 45 days; all rats were euthanized to collect blood and testicular samples on day 46. .e results showed that exposure of TCDD resulted in a decrease in SOD activity and live spermatozoa and increased MDA level and death, apoptosis, and necrosis of spermatozoa (T0) compared to the control (C) group (p< 0.05). .e administration of α-tocopherol, starting from the doses of 77 (T1), 149 (T2), and 259 mg (T3) per kg BW, was sequentially followed by returning MDA levels, recovering SOD activities, and restoration in the percentage of living, dead, apoptotic, and necrotic spermatozoa, similar (p> 0.05) to those of the control group. It could be concluded that the administration of α-tocopherol resolves the harmful e7ects of TCDD on the viability of spermatozoa in rats as a model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | - |
| Subjects: | Medicine |
| Divisions: | Journal Publication |
| Depositing User: | F.X. Hadi |
| Date Deposited: | 28 Nov 2025 02:54 |
| Last Modified: | 28 Nov 2025 02:54 |
| URI: | https://repositori.ukwms.ac.id/id/eprint/44814 |
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