Wihanto, Laura and Suyono, Handi (2021) Effects of Ethanol-based Antiseptic Solutions Against Gram Negative Bacteria. Jurnal Widya Medika, 7 (1). pp. 38-46. ISSN eISSN 2623-2723, pISSN: 2338-0373
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Abstract
Introduction: Gram-negative bacteria infections cause diseases, namely skin infection until sepsis, including nosocomial infection. Prevention by antiseptic application is the way to inhibit infection. Some antiseptic compounds that have been used show resistance according to some reports. Aim: Determine the effects of ethanol-based antiseptic solutions against Gram-negative bacteria. Methods: Discs saturated with ethanol-based antiseptic solutions were affixed to Muller Hinton agar smeared by Gram-negative bacteria such as Acinetobacter baumannii ATCC BAA-747, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC BAA-1706, Proteus vulgaris ATCC 6380, Pseudomonas aeruginosa ATCC 27853, and Salmonella sp. The diameter of the inhibition zone was read after 24 hours. Solutions are considered sensitive if the inhibition zone of growth diameter is more than 6 millimeters (Kirby-Bauer disc diffusion method). Result: Acinetobacter baumannii and Salmonella sp are sensitive to a solution consisting of ethanol 80 % and ethanol 80 %+ H2O2 0.15%. Pseudomonas aeruginosa and Escherichia coli are sensitive only to a solution consisting of ethanol 80 %+ H2O2 0,15%. As for Klebsiella pneumonia and Proteus vulgaris, they are resistant to both solutions. Conclusion: The use of ethanol-based antiseptic solutions with or without H2O2 0.15% addition does not effectively eliminate all gram-negative bacteria from the surface. The addition of 0.15% H2O2 to the antiseptic solution showed a better barrier effect than the solution containing only 80% ethanol. Adding other additives needs to be investigated further to formulate a better antiseptic solution against Gram-negative bacteria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Ethanol, Antiseptic, Gram-Negative Bacteria |
| Subjects: | Medicine |
| Divisions: | Journal Publication |
| Depositing User: | F.X. Hadi |
| Date Deposited: | 13 Aug 2025 02:52 |
| Last Modified: | 13 Aug 2025 02:52 |
| URI: | https://repositori.ukwms.ac.id/id/eprint/44372 |
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